TransStart® Top Green qPCR SuperMix is a ready-to-use qPCR cocktail containing a novel TransStart® Taq DNA Polymerase,optimized double cation buffer, SYBR Green I fluorescence dye, dNTPs, PCR Enhancer and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and H2O.
– TransStart ® Taq DNA Polymerase, a novel ‘hot start’ enzyme with double blocking technique, provides high sensitivity, high specificity and accurate data.
– Double cation (K + , NH 4 + ) buffer enhances the specificity and reduces primer-dimer formation.
– Passive reference dyes are suitable for different qPCR instruments (normalize the fluorescent signal between reactions).