TransStart® Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing a novel TransStart® Taq DNA Polymerase, optimized double cation buffer, dNTPs, PCR Enhancer and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, probe, passive reference dye (optional) and H2O. This kit takes advantage of using fluorescent probe (TaqMan or Molecular Beacon) in PCR reaction system. During the procedure of PCR amplification, the level of fluorescence signal is proportional to the amount of amplified products, thus the amount of nucleic acid in samples can be assessed by the intensity of fluorescence signal.
– TransStart ® Taq DNA Polymerase, a novel ‘hot start’ enzyme with double blocking technique, provides high sensitivity, high specificity and accurate data.
– Double cation (K + , NH 4 + ) buffer enhances the specificity and reduces primer-dimer formation.
– Passive reference dyes are suitable for different qPCR instruments (normalize the fluorescent signal between reactions).