TransStart® Green qPCR SuperMix UDG is a ready-to-use qPCR cocktail containing a novel TransStart® Taq DNA Polymerase, optimized double cation buffer, SYBR Green I fluorescence dye, dATP, dUTP,dCTP, dGTP, PCR Enhancer, PCR stabilizer and UDG (Uracil-DNA Glycosylase). qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template,primer, passive reference dye (optional) and H2O.
– TransStart ® Taq DNA Polymerase, a novel ‘hot start’ enzyme with double blocking technique, provides high sensitivity, high specificity and accurate data.
– Use of UDG enzyme and dUTP can effectively prevent cross-contamination from PCR product.
– Double cation (K + , NH4 + ) buffer enhances the specificity and reduces primer-dimer formation.
– Passive reference dyes are suitable for different qPCR instruments(normalize the fluorescent signal between reactions).