TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The supermix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, and not for qPCR.
– One-tube format for simple and fast setup and reduce pipetting variability.
– The optimal ratio of Oligo(dT)18 primer to random primer(N9) for PCR ready cDNA.
– PCR ready cDNA in 30 minutes (unsuitable for qPCR).
– cDNA up to 12 kb.