The Cell Biolabs Total Carbohydrate Assay detects total carbohydrates within samples based on the phenol-sulfuric acid method. Carbohydrates are hydrolyzed to furfural and furfural derivates upon addition of sulfuric acid, which then react with the Developing Solution to form a chromagen that is detected at 490nm. Total carbohydrate levels in an unknown sample are calculated based on a glucose standard curve.
- Colorimetric detection of total carbohydrates in serum, plasma, lysates, urine, cell culture supernatants or food samples
- Detection sensitivity limit of approximately 0.0625 mM glucose
- Glucose standard included