Thermostable RNA Ligase I is an ATP-dependent ligase that catalyzes intra- and intermolecular ligation of single-stranded RNA (ssRNA) molecules having a 5´-phosphate and a 3´-hydroxyl group at 60 ~65°C. This ligase also ligates ssDNA molecules.
- RNA-Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE).
- Ligation of ssRNA molecules.
- Ligation of adaptors to ssRNA or ssDNA.
- 5´-ligation tagging of RNA molecules.
Purified from a recombinant E. coli strain carrying a Thermovibrio ammonificans RNA Ligase 1 gene
- Thermostable RNA Ligase 1, 100 U/µl in storage buffer: 50 mM KCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5 @ 25°C
- 10 X Thermostable RNA Ligase 1 Buffer: 100 mM Bis-Tris-Propane-HCl, 100 mM MgCl2, 10 mM DTT, 0.5 mM ATP, pH 7 @ 25°C
1 unit of Thermostable RNA Ligase catalyzes the conversion of 10 pmole of a linear 5´-phosphorylated poly(A)18 to a phosphatase resistant form in one hour at 60°C under standard assay conditions
The absence of endo-, exodeoxyribonucleases, phosphatase and ribonucleases confirmed by appropriate quality tests.