Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.
This Protease inhibitor cocktail EDTA free is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
Protease inhibitor cocktail EDTA free has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.
Protocol: Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
|Contents||1 vial supplied at requested quantity.|
|Shipping Condition||Wet Ice|
|Storage||The product should be stored at -20°C, and is stable for at least 12 months.|