The OxiSelect™ Aldehyde-Induced DNA Damage ELISA Kit (Ethenocytidine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenocytidine antibody is added and binds competitively to ethenocytidine in the unknown sample or to the ethenocytidine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenocytidine in the sample.
- Detect as little as 625 nM of ethenocytidine
- Suitable for use with DNA isolated from cells or tissues
- Unknown samples are compared with a provided ethenocytidine standard