The Cell Biolabs LCAT Activity Assay Kit utilizes a fluorogenic dual-labeled phosphatidycholine as an LCAT substrate. When uncleaved, the fluorophores in the dual-labeled substrate are in a quenched state; upon hydrolysis by LCAT at the sn-2 position, fluorescent monomer chains are produced which can be measured in a fluorescence microplate reader (Ex. 342 nm/Em. 400 nm) (See Assay Principle).
The Lecithin:cholesterol acyltransferase (LCAT) Activity Assay Kit is a simple, fluorometric assay that quantitatively measures LCAT phospholipase activity in plasma, serum, and lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks and unknown samples. Besides LCAT, this assay can also be used to detect other calcium independent phospholipase activities such as lipoprotein phospholipase A2 (LP-PLA2).
- Quantitatively measures LCAT phospholipase activity in plasma, serum, lysates, as well as detect other calcium independent phospholipase activities
- Simple, fluorometric assay that can perform up to 100 assays