The Cell Biolab Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. A fluorometric probe is oxidized by hydrogen peroxide to generate fluorescent Resorufin, which correlates to the level of glutamate in the sample. Glutamate levels in an unknown sample are calculated based on a glutamate standard curve.
Glutamate is a non-essential amino acid that serves as an important neurotransmitter in the mammalian brain and has a key role in cellular metabolism. Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases.
- Detection sensitivity limit of 0.3 µM glutamate
- Suitable for use with lysates, cell culture supernatants, serum, plasma, and urine
- Glutamate standard included