TransGen FlyCut™ EcoRI-HF is an advanced High Fidelity and Fast restriction enzyme that is 100% active in the Universal Buffer and allows for rapid single-, double- or multiple DNA digestions in 5 minutes without the need for buffer changes or subsequent DNA clean-up steps.
TransGen FlyCut™ EcoRI-HF restriction enzyme recognises G^AATTC sites and cuts best at 37°C in 5 minutes using the Universal Buffer.
5′ G ↓ A A T T C 3′
3′ C T T A A↑ G 5′
• 100% activity of all FlyCut™ enzymes in the Universal Buffer
• Rapid digestion in 5 minutes
• No star activity
• One buffer for all restriction enzymes
• Molecular cloning
• Restriction site mapping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis