Name: BlueRAY Prestained Protein Ladder
SKU: PM006
Availability: InStock

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Unit Size

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PM006-0500

500 µl

$130.00

PM006-0500E

5× 500 µl

$580.00

Description
Additional information
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Description

The BlueRAY Prestained Protein Ladder is a three-colour protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is Ready-to-use. Do not heat, dilute, add reducing agent before loading.

– Broad range: 10-180 kDa.
– Ready-to-use: supplied in a loading buffer for direct loading on gels
– Easy to identify: includes a 25 kDa green band and 75 kDa red band for reference.
– Sharp bands.

Additional information

Unit Size	5× 500 µl, 500 µl
Size Range	10-180 kDa
Number of Bands	10
Recommended Load	3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2-3 µl per well for general Western transfer. – Apply more for thicker (>1.5 mm) or larger gel.
Storage Conditions	Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Storage Buffer	Approximately 0.2-0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) glycerol).